An Infrared Standard Curve Method for Calculating the Total Amount of Petroleum in Environmental Samples
-
摘要:
石油类是环境监测必测指标之一,主要以烃类形式存在,所以红外分光光度法是公认测定石油类总量最可靠的检测方法。红外分光光度法测定石油类总量目前有三波数的校正系数法和非色散的单波数标准曲线法。校正系数法计算步骤多、工作量大;单波数标准曲线法虽然简单,但是文献中有的未明确使用哪个波数进行计算,有的由于计算结果偏差较大而被限制适用范围或被直接舍弃。本文针对标准曲线法计算石油类总量的方法开展研究,通过三个波数处吸光度建立了5种标准曲线法,并对原油、高温润滑油、机油、0#柴油和92#汽油五种配制油品进行计算结果比对,确定了三波数吸光度之和标准曲线法是5种标准曲线法中的最佳方法。用芳香烃占比试验考察了油品适用范围,并进行了样品加标实验和实际样品实验验证其实用性,结果表明在芳香烃占比小于50%时。该方法精密度为5.9%~8.0%,加标回收率为76.4%~98.2%,满足环境标准要求。该方法拓展了标准曲线法的适用范围、简单易懂、操作性强,是现行校正系数法的有益补充。
-
关键词:
- 环境样品 /
- 石油类总量 /
- 红外分光光度法 /
- 三波数之和标准曲线法
Abstract:BACKGROUND Petroleum oil is one of the necessary indicators for environmental monitoring, which mainly exists in the form of hydrocarbons. Therefore, infrared spectrophotometry is one of the most reliable methods to determine the total amount of petroleum oil. At present, there are two methods for the determination of petroleum by infrared spectrophotometry, which are the three-wave number correction coefficient method and the non-dispersive single wave number standard curve method. The correction coefficient method has many calculation steps and a heavy workload; the single wave number standard curve method does not specify which wave number to use for calculation or is abandoned due to the limited scope of application.
OBJECTIVES To establish a simple standard curve method for calculating the total amount of petroleum.
METHODS Five kinds of oils were scanned by Fourier transform infrared (FTIR) spectroscopy. The absorbance of the three scanning wavenumbers was arranged and combined to obtain five standard curve methods. After comparing the calculated concentration with the prepared concentration, the standard curve method of the sum of three-wave number absorbance was established.
RESULTS When the proportion of aromatic hydrocarbons was less than 50%, the calculation results of this method were consistent with those of the correction coefficient method. The precision of this method was 5.9%-8.0%, and the recovery rate of standard addition was 76.4%-98.2%, which meets the requirements of China's petroleum pollution determination and environmental standards.
CONCLUSIONS This method expands the application scope of the standard curve method. It is simple and easy to understand and has strong operability. It is a useful supplement to the correction coefficient method.
-
-
表 1 标准曲线方程与相关系数
Table 1. Standard curve equations and correlation coefficients
标准曲线名称 回归方程 相关系数(R) 2930cm-1标准曲线 y=0.0135x+0.015 0.9996 2960cm-1标准曲线 y=0.0078x-0.0041 0.9998 3030cm-1标准曲线 y=0.0011x-0.0013 0.9996 两波数吸光度之和标准曲线 y=0.0214x-0.008 0.9998 三波数吸光度之和标准曲线 y=0.0225x-0.0065 0.9999 表 2 芳香烃占比试验结果
Table 2. Results of the proportion test for aromatic hydrocarbons
三种烃比例(正十六烷∶异辛烷∶苯) 芳香烃占比(%) 配制浓度(mg/L) 三波数之和标准曲线法 计算值(mg/L) 回收率(%) 7 ∶ 3 ∶ 0 0 50.00 59.98 119.96 6 ∶ 3 ∶ 1 10 50.00 55.42 110.83 6 ∶ 2 ∶ 2 20 50.00 52.93 105.86 5 ∶ 2 ∶ 3 30 50.00 46.70 93.39 5 ∶ 1 ∶ 4 40 50.00 43.66 87.32 3 ∶ 2 ∶ 5 50 50.00 36.55 73.10 3 ∶ 1 ∶ 6 60 50.00 33.47 66.94 2 ∶ 1 ∶ 7 70 50.00 28.24 56.48 1 ∶ 1 ∶ 8 80 50.00 22.43 44.86 1 ∶ 0 ∶ 9 90 50.00 18.65 37.29 0 ∶ 0 ∶ 10 100 50.00 13.85 27.69 表 3 空白加标样品精密度结果
Table 3. Precision results of blank spiked samples
测定次数 土壤空白加标样品石油类物质含量(mg/kg) 水质空白加标样品石油类物质含量(mg/kg) 10mg/kg 50mg/kg 100mg/kg 0.10mg/L 0.50mg/L 2.50mg/L 1 9.11 47.6 93.4 0.0823 0.458 2.36 2 7.93 48.3 92.5 0.0764 0.403 2.13 3 9.29 47.4 91.6 0.0951 0.471 2.08 4 8.26 49.1 94.1 0.0876 0.427 2.41 5 8.74 45.7 95.7 0.0811 0.452 2.24 6 7.73 48.2 90.6 0.0798 0.485 2.33 平均值 8.51 47.7 93 0.0837 0.45 2.26 回收率(%) 77.3~92.9 91.4~98.2 91.6~95.7 76.4~95.1 80.6~97.0 83.2~96.4 RSD(%) 7.5 6.7 5.9 8.0 6.7 5.9 表 4 实际样品的计算结果对比
Table 4. Comparison of calculation results for actual samples
土壤样品编号 土壤样品中石油类物质含量(mg/kg) 水样品编号 水样品中石油类物质含量(mg/L) 校正系数法
(标准方法)三波数之和标准曲线法
(本文方法)相对偏差
(%)校正系数法
(标准方法)三波数之和标准曲线法
(本文方法)相对偏差
(%)T-1 17.4 18.5 -3.1 S-1 0.08 0.07 6.7 T-2 9.73 9.82 -0.5 S-2 0.11 0.12 -4.3 T-3 87.9 90.9 -1.7 S-3 0.09 0.1 -5.3 T-4 104 94.8 4.6 S-4 0.67 0.65 1.5 T-5 374 393 -2.5 S-5 0.88 0.92 -2.2 T-6 646 689 -3.2 S-6 0.79 0.84 -3.1 T-7 1235 1304 -2.7 S-7 1.25 1.18 2.9 T-8 1647 1723 -2.3 S-8 1.34 1.26 3.1 T-9 5386 5839 -4.0 S-9 1.87 1.67 5.6 T-10 20880 22342 -3.4 S-10 2.07 2.14 -1.7 注:相对偏差=(推荐方法测定值-两次测定值的平均值)/两次测定值平均值×100%。 -
[1] 李国欣, 雷征东, 董伟宏, 等. 中国石油非常规油气开发进展、挑战与展望[J]. 中国石油勘探, 2022, 27(1): 1-11. https://www.cnki.com.cn/Article/CJFDTOTAL-KTSY202201001.htm
Li G X, Lei Z D, Dong W H, et al. China's oil reserves progress, challenges and prospects of unconventional oil and gas development of CNPC[J]. China Petroleum Exploration, 2022, 27(1): 1-11. https://www.cnki.com.cn/Article/CJFDTOTAL-KTSY202201001.htm
[2] Ahmed O E, Mahmoud S A, Mousa A E M. Aliphatic and poly-aromatic hydrocarbons pollution at the Drainage Basin of Suez Oil Refinery Company[J]. Current Science International, 2015, 4(1): 27-44.
[3] 李照, 许玉玉, 张世凯, 等. 海洋溢油污染及修复技术研究进展[J]. 山东建筑大学学报, 2020, 35(6): 69-75. https://www.cnki.com.cn/Article/CJFDTOTAL-SDJG202006011.htm
Li Z, Xu Y Y, Zhang S K, et al. Research progress of marine petroleum pollution and remediation technology[J]. Journal of Shandong Jianzhu University, 2020, 35(6): 69-75. https://www.cnki.com.cn/Article/CJFDTOTAL-SDJG202006011.htm
[4] 魏样. 土壤石油污染的危害及现状分析[J]. 中国资源综合利用, 2020, 38(4): 120-122. doi: 10.3969/j.issn.1008-9500.2020.04.033
Wei Y. The harm and present situation of soil oil pollution[J]. China Resources Comprehensive Utilization, 2020, 38(4): 120-122. doi: 10.3969/j.issn.1008-9500.2020.04.033
[5] 张道来, 刘娜, 朱志刚, 等. 山东半岛典型海岸带多环芳烃分布特征、来源解析及风险评价[J]. 岩矿测试, 2016, 35(5): 521-529. doi: 10.15898/j.cnki.11-2131/td.2016.05.011 http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.2016.05.011
Zhang D L, Liu N, Zhu Z G, et al. Distribution, sources and risk assessment of polycyclic aromatic hydrocarbons in surface sediments from typical coast of Shandong Peninsulia[J]. Rock and Mineral Analysis, 2016, 35(5): 521-529. doi: 10.15898/j.cnki.11-2131/td.2016.05.011 http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.2016.05.011
[6] 曹小聪, 吴晓晨, 徐文帅, 等. 水和沉积物中石油烃的分析方法及污染特征研究进展[J]. 环境工程技术学报, 2020, 10(5): 871-882. https://www.cnki.com.cn/Article/CJFDTOTAL-HKWZ202005028.htm
Cao X C, Wu X C, Xu W S, et al. Research progress of analytical methods and pollution characteristics of petroleum hydrocarbons in water and sediment[J]. Journal of Environmental Engineering Technology, 2020, 10(5): 871-882. https://www.cnki.com.cn/Article/CJFDTOTAL-HKWZ202005028.htm
[7] 刘丹青. 我国污染场地土壤石油烃环境质量标准体系的现状与趋势[J]. 中国环境监测, 2020, 36(1): 138-146. https://www.cnki.com.cn/Article/CJFDTOTAL-IAOB202001025.htm
Liu D Q. Current situation and trend of petroleum hydrocarbon related standard system in contaminated site soils of China[J]. Environmental Monitoring in China, 2020, 36(1): 138-146. https://www.cnki.com.cn/Article/CJFDTOTAL-IAOB202001025.htm
[8] 黄勇, 王安婷, 袁国礼, 等. 北京市表层土壤中PAHs含量特征及来源分析[J]. 岩矿测试, 2022, 41(1): 54-65. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.202104270056
Huang Y, Wang A T, Yuan G L, et al. The content characteristics and source analysis of polycyclic aromatic hydrocarbons in the topsoil of Beijing City[J]. Rock and Mineral Analysis, 2022, 41(1): 54-65. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.202104270056
[9] 张厚福. 石油地质学[M]. 北京: 石油工业出版社, 1999: 20.
Zhang H F. Petroleum geology[M]. Beijing: Petroleum Industry Press, 1999: 20.
[10] Adeniji A O, Okoh O O, Okoh A I. Analytical methods for the determination of the distribution of total petroleum hydrocarbons in the water and sediment of aquatic systems: A review[J]. Journal of Chemistry, 2017: 1-13.
[11] 吴嘉鹏, 楼振纲, 胡笑妍, 等. 紫外法与红外法测定石油类的比对研究[J]. 中国无机分析化学, 2019, 9(6): 78-82. https://www.cnki.com.cn/Article/CJFDTOTAL-WJFX201906017.htm
Wu J P, Lou Z G, Hu X Y, et al. Comparison of ultraviolet and infrared spectrophotometry in the determination of petroleum[J]. Chinese Journal of Inorganic Analytical Chemistry, 2019, 9(6): 78-82. https://www.cnki.com.cn/Article/CJFDTOTAL-WJFX201906017.htm
[12] 段小燕, 管雪丽, 吐拉别克·吐逊江. 测定地表水中石油类的方法比较[J]. 干旱环境监测, 2021, 35(4): 167-170. https://www.cnki.com.cn/Article/CJFDTOTAL-GHJC202104005.htm
Duan X Y, Guan X L, Tulabieke T X J. Comparison of methods for determining petroleum oils in surface water[J]. Arid Environmental Monitoring, 2021, 35(4): 167-170. https://www.cnki.com.cn/Article/CJFDTOTAL-GHJC202104005.htm
[13] 赵昌平, 冯小康, 朱强. 快速溶剂萃取-气相色谱法测定土壤中石油烃(C10~C40)[J]. 理化检验(化学分册), 2020, 56(7): 827-831. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH202007020.htm
Zhao C P, Feng X K, Zhu Q. GC determination of petroleum hydrocarbons (C10-C40) in soil with rapid solvent extraction[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2020, 56(7): 827-831. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH202007020.htm
[14] 曹攽, 胡祖国, 郑存江, 等. 超声萃取-气相色谱法测定土壤中石油烃[J]. 理化检验(化学分册), 2018, 54(3): 275-279. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH201803008.htm
Cao B, Hu Z G, Zheng C J, et al. Determination of petroleum hydrocarbons in soil by GC combined with ultrasonic extraction[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2018, 54(3): 275-279. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH201803008.htm
[15] 刘玉龙, 黄燕高, 刘菲. 气相色谱法测试土壤中分段石油烃的标准化定量方法初探[J]. 岩矿测试, 2019, 38(1): 102-111. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.201709040139
Liu Y L, Huang Y G, Liu F. Analysis of total petroleum hydrocarbon fractions in soils by gas chromatography: Standardized calibration and quantitation method[J]. Rock and Mineral Analysis, 2019, 38(1): 102-111. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.201709040139
[16] 段旭, 李慧慧, 杨柳晨, 等. 土壤中总石油烃测定——3种前处理方法的对比[J]. 福建分析测试, 2019, 28(3): 47-50. https://www.cnki.com.cn/Article/CJFDTOTAL-FJFC201903016.htm
Duan X, Li H H, Yang L C, et al. Three pretreatment methods of determination of total petroleum hydrocarbon in soil[J]. Fujian Analysis & Testing, 2019, 28(3): 47-50. https://www.cnki.com.cn/Article/CJFDTOTAL-FJFC201903016.htm
[17] 韩彬, 林法祥, 丁宇, 等. 海州湾近岸海域水质状况调查与风险评价[J]. 岩矿测试, 2019, 38(4): 429-437. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.201806190073
Han B, Lin F X, Ding Y, et al. Quality survey and risk assessment of the coastal waters of Haizhou Bay[J]. Rock and Mineral Analysis, 2019, 38(4): 429-437. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.201806190073
[18] 赵江华, 王鹏, 黎卫亮, 等. 复垦土地样品中石油类物质加速溶剂萃取-荧光分光光度法分析方法研究[J]. 岩矿测试, 2021, 40(3): 375-383. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.202011200150
Zhao J H, Wang P, Li W L, et al. Determination of the petroleum substances in samples of reclaimed land by fluorescence spectrophotometry with accelerated solvent extraction[J]. Rock and Mineral Analysis, 2021, 40(3): 375-383. http://www.ykcs.ac.cn/cn/article/doi/10.15898/j.cnki.11-2131/td.202011200150
[19] 薛广海, 李强, 刘庆, 等. 当前国内外含油污泥处理标准及石油烃检测方法的深度剖析和对比[J]. 石油化工应用, 2019, 38(1): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-NXSH201901001.htm
Xue G H, Li Q, Liu Q, et al. In-depth analysis and comparison on the standards and testing methods for oil contaminated soil of domestic and international[J]. Petrochemical Industry Application, 2019, 38(1): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-NXSH201901001.htm
[20] 韩文靖, 王小书. 表面活性剂淋洗技术在含油土壤污染治理中的应用[J]. 当代化工, 2021, 50(3): 558-561, 566. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHH202103025.htm
Han W J, Wang X S. Research on the application of surfactant leaching technology in the treatment of oily soil pollution[J]. Contemporary Chemical Industry, 2021, 50(3): 558-561, 566. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHH202103025.htm
[21] 苏丽娜, 马晓利, 陈平. 低含量油污染土壤中总石油烃测定萃取方法研究[J]. 应用化工, 2017, 46(8): 1635-1639. https://www.cnki.com.cn/Article/CJFDTOTAL-SXHG201708046.htm
Su L N, Ma X L, Chen P. Study on extraction and determination of total petroleum hydrocarbons in low oil-contaminated soil[J]. Applied Chemical Industry, 2017, 46(8): 1635-1639. https://www.cnki.com.cn/Article/CJFDTOTAL-SXHG201708046.htm
[22] 马晓利, 苏丽娜, 庞林, 等. 快速溶剂萃取-红外分光光度法测定低含量油污染土壤中总石油烃的含量[J]. 理化检验(化学分册), 2018, 54(4): 388-392. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH201804004.htm
Ma X L, Su L N, Pang L, et al. Determination of total petroleum hydrocarbons in low oil-contaminated soil by infrared spectrophotometry with accelerated solvent extraction[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2018, 54(4): 388-392. https://www.cnki.com.cn/Article/CJFDTOTAL-LHJH201804004.htm
[23] 王玉纯, 林大泉. 用红外分光光度法测定水体中石油烃含量的研究[J]. 石油化工环境保护, 1988(2): 53-57. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHB198802015.htm
Wang Y C, Lin D Q. Determination of petroleum hydrocarbon content in water by infrared spectrophotometry[J]. Petrochemical Environmental Protection, 1988(2): 53-57. https://www.cnki.com.cn/Article/CJFDTOTAL-SYHB198802015.htm
[24] 刘廷良, 刘京, 齐文启, 等. 水中石油类分析方法的现状[J]. 环境科学研究, 2000, 13(5): 58-60. https://www.cnki.com.cn/Article/CJFDTOTAL-HJKX200005021.htm
Liu T L, Liu J, Qi W Q, et al. Introduction of oil analysis methods for water and wastewater[J]. Research of Environmental Sciences, 2000, 13(5): 58-60. https://www.cnki.com.cn/Article/CJFDTOTAL-HJKX200005021.htm
[25] 杨斌, 刘景龙, 郭艳. 红外分光光度法测定石油类中标准溶液稳定性的探讨[J]. 广州化学, 2020, 45(5): 65-68. https://www.cnki.com.cn/Article/CJFDTOTAL-GZHX202005011.htm
Yang B, Liu J L, Guo Y. Determination of mercury in water by atomic fluorescence spectrometry under the different working temperature[J]. Guangzhou Chemistry, 2020, 45(5): 65-68. https://www.cnki.com.cn/Article/CJFDTOTAL-GZHX202005011.htm
[26] 梁庆勋, 叶洋宏. 红外分光光度法测定水中石油类手工分析方式与全自动分析方式比对研究[J]. 广东化工, 2021, 48(6): 127-128. https://www.cnki.com.cn/Article/CJFDTOTAL-GDHG202106063.htm
Liang Q X, Ye Y H. Comparison of manually and automatic mode for determination of petroleum pollutants in water by infrared spectrophotometry[J]. Guangdong Chemical Industry, 2021, 48(6): 127-128. https://www.cnki.com.cn/Article/CJFDTOTAL-GDHG202106063.htm
[27] 马宏伟. 红外分光光度法测定水中石油类探讨[J]. 环境科学导刊, 2011, 30(1): 87-88. https://www.cnki.com.cn/Article/CJFDTOTAL-YNHK201101024.htm
Ma H W. Study on the determination of petroleum oil in the water[J]. Environmental Science Survey, 2011, 30(1): 87-88. https://www.cnki.com.cn/Article/CJFDTOTAL-YNHK201101024.htm
[28] 杨丹丹, 姚亮, 雷世勇. 《水质石油类和动植物油的测定》新旧标准比较[J]. 环境科学与管理, 2013, 38(4): 116-119. https://www.cnki.com.cn/Article/CJFDTOTAL-BFHJ201304026.htm
Yang D D, Yao L, Lei S Y. Comparison between the new and old version of "Water Quality-Determination of petroleum oils and animal and vegetable oils"[J]. Environmental Science and Management, 2013, 38(4): 116-119. https://www.cnki.com.cn/Article/CJFDTOTAL-BFHJ201304026.htm
-