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黑龙江林甸地热田热水微生物多样性与群落结构分析

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文章导航 > 中国地质 > 2023 > 50(6): 1667-1677

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朱瑞杰, 周学军, 詹涛, 马永法, 王旭, 董俊领, 刘玲, 杨峰田, 李栋, 石宇佳, 苏玉娟. 2023. 黑龙江林甸地热田热水微生物多样性与群落结构分析[J]. 中国地质, 50(6): 1667-1677. doi: 10.12029/gc20210602003
引用本文: 朱瑞杰, 周学军, 詹涛, 马永法, 王旭, 董俊领, 刘玲, 杨峰田, 李栋, 石宇佳, 苏玉娟. 2023. 黑龙江林甸地热田热水微生物多样性与群落结构分析[J]. 中国地质, 50(6): 1667-1677. doi: 10.12029/gc20210602003
shu
ZHU Ruijie, ZHOU Xuejun, ZHAN Tao, MA Yongfa, WANG Xu, DONG Junling, LIU Ling, YANG Fengtian, LI Dong, SHI Yujia, SU Yujuan. 2023. Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province[J]. Geology in China, 50(6): 1667-1677. doi: 10.12029/gc20210602003
Citation: ZHU Ruijie, ZHOU Xuejun, ZHAN Tao, MA Yongfa, WANG Xu, DONG Junling, LIU Ling, YANG Fengtian, LI Dong, SHI Yujia, SU Yujuan. 2023. Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province[J]. Geology in China, 50(6): 1667-1677. doi: 10.12029/gc20210602003
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黑龙江林甸地热田热水微生物多样性与群落结构分析

  • 1.

    地下水资源与环境教育部重点实验室 (吉林大学), 吉林 长春 130021

  • 2.

    吉林省水资源与水环境重点实验室 (吉林大学), 吉林 长春 130021

  • 3.

    黑龙江省生态地质调查研究院, 黑龙江 哈尔滨 150030

  • 基金项目:
    本文由国家自然科学青年基金项目（41202167）资助
详细信息
    作者简介: 朱瑞杰, 男, 1998年生, 硕士生, 主要从事地质地热研究; E-mail: zhurj20@mails.jlu.edu.cn
    通讯作者: 苏玉娟, 女, 1980年生, 高级工程师, 主要从事地热资源评价和开发利用研究; E-mail: suyj15@mails.jlu.edu.cn

  • 中图分类号: Q938;P314

Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province

  • 1.

    Key Laboratory of Groundwater Resources and Environment(Jilin University), Ministry of Education, Changchun 130021, Jilin, China

  • 2.

    Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun 130021, Jilin, China

  • 3.

    The Institute of Ecological Geology Survey and Research of Heilongjiang Province, Harbin 150030, Heilongjiang, China

  • Fund Project: Supported by the National Natural Science Foundation (Youth) (No.41202167)
More Information
    Author Bio: ZHU Ruijie, male, born in 1998, master candidate, mainly engaged in geology and geothermal research; E-mail: zhurj20@mails.jlu.edu.cn .
    Corresponding author: SU Yujuan, female, born in 1980, senior engineer, mainly engaged in genesis, development and utilization of geothermal resources; E-mail: suyj15@mails.jlu.edu.cn

    摘要
  • 研究目的

    研究地热系统微生物群落组成有助于揭示地热流体地球化学演化和指示热储地球化学环境。已有研究显示，林甸地热田热水还原性强且富含甲烷，但关于微生物在甲烷形成中的作用研究较少。本文旨在分析林甸地热田热水中微生物的群落结构和多样性特征，并揭示其甲烷生成途径。

     研究方法

    在地热供暖季和非供暖季采集并测试了9个热水微生物样品，并对热水中微生物多样性、群落结构及功能基因进行了分析。

     研究结果

    属水平上，林甸地热水中优势细菌主要为不动杆菌属（Acinetobacter，>80%），与已有报道的地热系统有一定差异，而同油田热水相似；供暖季和非供暖季热水中细菌多样性差异较大，非供暖季细菌多样性高于供暖季，但古菌多样性基本不受开采影响；优势古菌以广古菌门（Euryarchaeota）产甲烷菌为主，地热流体中的甲烷气体主要源于H2还原CO2产甲烷途径，而甲基歧化产甲烷作用和H2还原甲基化合物产甲烷途径次之。

     结论

    林甸地热田热水中微生物多样性和群落结构较为独特，与地层中有机质含量较高、地热开采扰动有关。

    This paper is the result of geothermal survey engineering.

     Objective

    Researches on microbial community in geothermal systems is key to indicate geochemical environment and evolution of thermal fluids. Previous researches have shown that the thermal water in Lindian geothermal field is highly reducing and is rich in methane. The objective of this paper is to characterize the microbial diversity and community structure of the thermal water.

     Methods

    A total of 9 thermal water samples were collected and tested during exploitation and non-exploitation periods, and the microbial diversity, community structure and functional genes of the samples were analyzed.

     Results

    The dominant bacterial genera in the thermal water is mainly Acinetobacter, which is different from most geothermal systems reported, but are similar to that of oil fields. The bacterial diversity of the thermal waters during the exploitation period and the non-exploitation period is significantly different, i.e., the bacterial diversity in the non-exploitation period is higher than that in the exploitation period, while the archaeal diversity is not affected significantly by exploitation. The dominant archaea are mainly Euryarchaeota methanogens. Functional gene prediction of archaea shows that methane production in Lindian geothermal field is dominated by hydrogenotrophic methanogenesis, and secondarily by disproportionation of methyl groups and reduction of methyl compounds with H2.

     Conclusions

    The microbial diversity and community structure of the thermal waters from Lindian geothermal field is relatively unique, which is related to the high content of organic matters and thermal water exploitation.
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  • 图 1  林甸地热田热水微生物采样点分布

    Figure 1. 

    下载: 全尺寸图片 幻灯片

    图 2  细菌（a）和古菌（b）样品间Bray-Curtis距离热图

    Figure 2. 

    下载: 全尺寸图片 幻灯片

    图 3  林甸地热田热水细菌（门水平）相对丰度

    Figure 3. 

    下载: 全尺寸图片 幻灯片

    图 4  林甸地热田热水古菌（属水平）相对丰度

    Figure 4. 

    下载: 全尺寸图片 幻灯片

    图 5  古菌群落功能预测相对丰度热图

    Figure 5. 

    下载: 全尺寸图片 幻灯片

    表 1  采样井井深和热水水化学特征

    Table 1.  Depth of sampling wells and thermal water chemistry

    下载: 导出CSV

    表 2  样品中细菌群落多样性指标

    Table 2.  Diversity indices of bacterial communities in the investigated water samples

    下载: 导出CSV

    表 3  调查样品中古菌群落多样性指标

    Table 3.  Diversity indices of Archaeal communities in the investigated water samples

    下载: 导出CSV
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收稿日期:  2021-06-02
修回日期:  2022-03-03
刊出日期:  2023-12-25

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