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摘要:
全球甲烷排放主要来源于厌氧环境中产甲烷菌的代谢活动。采集渤海典型站位沉积物样品，测定了沉积物中甲烷和硫酸盐含量。通过提供不同类型产甲烷底物进行培养，分析了各样品中甲烷产量；通过高通量测序，分析了产甲烷菌群落特征。结果表明，渤海沉积物中产甲烷途径以甲基营养型为主，同时具有H2/CO2还原型途径。同一站点随着深度的增加，甲烷产生量逐渐减少，产甲烷速率相应降低。古菌群落以Crenarchaeota、Asgardaeota和Nanoarchaeota为主，产甲烷菌Ca. Methanofastidiosales占据优势地位。本研究为全面了解产甲烷菌在海洋生境中产甲烷过程提供了重要参考。
Abstract:Mostly, the global methane emission comes from the metabolic activities of methanogens, which occurs in anaerobic environment. Based on the samples collected from typical stations in the Bohai Sea, we measured the concentrations of methane and sulfate in the sediments. The methane concentration in each sample was analyzed with different methanogenic substrates cultivation. The methanogen communities were measured by high-throughput sequencing techniques. The results indicated that the mainly methylotrophic pathway is methylotrophic methanogenesis, along with the hydrogenotrophic methanogenesis. Methane production decreased gradually with the depth increasing at the same station, and methane production rate decreased correspondingly at the same time. The archaea communities were dominated by Crenarchaeota, Asgardaeota and Nanoarchaeota, and Ca. Methanofastidiosales
was the dominated methanogens. The results provide useful information for understanding the methanogenesis of methanogens in marine ecosystem. -
Key words:
- methanogens /
- sediment /
- methylotrophic methanogenesis /
- hydrogenotrophic methanogenesis /
- Bohai Sea
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表 1 培养基配方
Table 1. Composition of the medium
试剂名称 浓度/(g L−1) NH4Cl 0.53 KH2PO4 0.10 MgCl2·6H2O 4.00 CaCl2·2H2O 1.00 NaCl 25.00 NaHCO3 2.00 
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表 2 古菌群落多样性特征
Table 2. Diversity characteristics of archaea community
站位 深度/cm 序列 OTU个数 Shannon Simpson Chao1 Goods Coverage/% M1 2-6 13,657 428 3.84 0.054 468 99.41 20-24 31,748 486 4.04 0.042 491 99.93 M7 2-6 16,160 519 3.10 0.168 581 99.34 20-24 25,892 741 4.59 0.029 806 99.52 32-36 27,298 606 4.60 0.034 620 99.84
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